| Franchise | City | Founded | Titles |
|---|---|---|---|
| Atlanta United FC | Atlanta | 2014 | 1 |
| Austin FC | Austin | 2018 | — |
| CF Montréal | Montreal | 2010 | — |
| Charlotte FC | Charlotte | December 17, 2019 | — |
| Chicago Fire FC | Chicago | October 8, 1997 | 1 |
| Colorado Rapids | Commerce City | 1995 | — |
| Columbus Crew | Columbus | 1994 | 3 |
| D.C. United | Washington, D.C. | 1994 | 4 |
| FC Cincinnati | Cincinnati | January 27, 2017 | — |
| FC Dallas | Frisco | 1995 | 1 |
| Houston Dynamo FC | Houston | 2005 | 2 |
| Inter Miami CF | Miami | January 29, 2018 | 1 |
| LA Galaxy | Los Angeles | 1995 | 6 |
| Los Angeles FC | Los Angeles | 2014 | 1 |
| Minnesota United FC | Minneapolis | 2015 | — |
| Nashville SC | Nashville | 2017 | — |
| New England Revolution | Foxborough | 1995 | — |
| New York City FC | New York City | May 21, 2013 | 1 |
| Orlando City SC | Orlando | 2013 | — |
| Philadelphia Union | Chester | 2008 | — |
| Portland Timbers | Portland | 2009 | 1 |
| Real Salt Lake | Salt Lake City | 2005 | 1 |
| Red Bull New York | Harrison | 1995 | — |
| San Diego FC | San Diego | May 18, 2023 | — |
| San Jose Earthquakes | San Jose | 1995 | 2 |
| Seattle Sounders FC | Seattle | 2009 | 1 |
| Sporting Kansas City | Kansas City | 1995 | 2 |
| St. Louis City SC | St. Louis | August 20, 2019 | — |
| Toronto FC | Toronto | 2006 | 2 |
| Vancouver Whitecaps FC | Vancouver | 2009 | — |